解剖学和形态学
麻醉学
听力与言语-语言病理学
行为科学
心脏和心血管系统
细胞和组织工程学
临床神经病学
危重症监护医学
牙科,口腔外科和医学
皮肤病学
急诊医学
内分泌学和新陈代谢
肠胃学和肝脏学
老人病学和老年医学
卫生保健科学和服务
血液学
免疫学
传染病
综合和补充性医学
医学伦理学
医学信息学
医学实验室技术
医学,全科和内科
医学,法律
医学,研究和试验
神经系统科学
护理
营养学和饮食学
产科医学和妇科医学
肿瘤学
眼科学
整形外科学
耳鼻喉科学
病理学
儿科学
周围血管疾病
药理学和药剂学
生理学
基本医疗保健
精神病学
公共、环境和职业卫生
放射学,核医学和医学成像
康复学
生殖生物学
呼吸系统
风湿病学
运动科学
外科学
毒理学
热带医学
泌尿学和肾脏学
病毒学
老年医学
健康政策和服务
心理学,临床
abstract::Transgenic rice accumulating the modified major Japanese cedar pollen allergens, Cryptomeria japonica 1 (Cry j 1) and Cryptomeria japonica 2 (Cry j 2), which were deconstructed by fragmentation and shuffling, respectively, in the edible part of the seed was generated by transformation of a good-tasting rice variety, '...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/pbi.12007
更新日期:2013-01-01 00:00:00
abstract::High temperature impairs rice (Oryza sativa) grain filling by inhibiting the deposition of storage materials such as starch, resulting in mature grains with a chalky appearance, currently a major problem for rice farming in Asian countries. Such deterioration of grain quality is accompanied by the altered expression o...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/j.1467-7652.2012.00741.x
更新日期:2012-12-01 00:00:00
abstract::Multivitamin corn is a novel genetically engineered variety that simultaneously produces high levels of β-carotene, ascorbate and folate, and therefore has the potential to address simultaneously multiple micronutrient deficiencies caused by the lack of vitamins A, B9 and C in developing country populations. As part o...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/j.1467-7652.2012.00730.x
更新日期:2012-12-01 00:00:00
abstract::Mutation discovery technologies have enabled the development of reverse genetics for many plant species and allowed sophisticated evaluation of the consequences of mutagenesis. Such methods are relatively straightforward for seed-propagated plants. To develop a platform suitable for vegetatively propagated species, we...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/j.1467-7652.2012.00733.x
更新日期:2012-12-01 00:00:00
abstract::An important goal in biotechnological research is to improve the yield of crop plants. Here, we genetically modified simultaneously source and sink capacities in potato (Solanum tuberosum cv. Desirée) plants to improve starch yield. Source capacity was increased by mesophyll-specific overexpression of a pyrophosphatas...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/j.1467-7652.2012.00736.x
更新日期:2012-12-01 00:00:00
abstract::Drought conditions limit agricultural production by preventing crops from reaching their genetically predetermined maximum yields. Here, we present the results of field evaluations of rice overexpressing OsNAC9, a member of the rice NAC domain family. Root-specific (RCc3) and constitutive (GOS2) promoters were used to...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/j.1467-7652.2012.00697.x
更新日期:2012-09-01 00:00:00
abstract::A new understanding of leaf starch degradation has emerged in the last 10 years. It has been shown that starch phosphorylation and dephosphorylation are critical components of this process. Glucan, water dikinase (GWD) (and phosphoglucan, water dikinase) adds phosphate to starch, and phosphoglucan phosphatase (SEX4) r...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/j.1467-7652.2012.00684.x
更新日期:2012-06-01 00:00:00
abstract::Broad spectrum protection against different insects and pathogens requires multigene engineering. However, such broad spectrum protection against biotic stress is provided by a single protein in some medicinal plants. Therefore, tobacco chloroplasts were transformed with the agglutinin gene from Pinellia ternata (pta)...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/j.1467-7652.2011.00663.x
更新日期:2012-04-01 00:00:00
abstract::A high-amylose rice with 64.8% amylose content (AC) was developed by transgenic inhibition of two isoforms of starch branching enzyme (SBE), SBEI and SBEIIb, in an indica rice cultivar. The expression of SBEI and SBEIIb was completely inhibited in the transgenic line, whereas the expression of granule-bound starch syn...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/j.1467-7652.2011.00667.x
更新日期:2012-04-01 00:00:00
abstract::Although a physiological role of heat-shock proteins (HSP) in antigen presentation and immune response activation has not been directly demonstrated, their use as vaccine components is under clinical trial. We have previously demonstrated that the structure of plant-derived HSP70 (pHSP70) can be superimposed to the ma...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/j.1467-7652.2011.00673.x
更新日期:2012-04-01 00:00:00
abstract::Heavy metal accumulation in the environment poses great risks to flora and fauna. However, monitoring sites prone to accumulation poses scale and economic challenges. In this study, we present and test a method for monitoring these sites using fluorescent resonance energy transfer (FRET) change in response to zinc (Zn...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/j.1467-7652.2011.00656.x
更新日期:2012-02-01 00:00:00
abstract::The rice α-amylase 3D promoter system, which is activated under sucrose-starved conditions, has emerged as a useful system for producing recombinant proteins. However, using rice as the production system for therapeutic proteins requires modifications of the N-glycosylation pattern because of the potential immunogenic...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/j.1467-7652.2011.00636.x
更新日期:2011-12-01 00:00:00
abstract::Starch is an important renewable raw material with an increasing number of applications. Several attempts have been made to obtain plants that produce modified versions of starch or higher starch yield. Most of the approaches designed to increase the levels of starch have focused on the increment of the amount of ADP-...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/j.1467-7652.2011.00626.x
更新日期:2011-12-01 00:00:00
abstract::Food security is a global concern and substantial yield increases in cereal crops are required to feed the growing world population. Wheat is one of the three most important crops for human and livestock feed. However, the complexity of the genome coupled with a decline in genetic diversity within modern elite cultiva...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/j.1467-7652.2011.00628.x
更新日期:2011-12-01 00:00:00
abstract::Drought is the major environmental factor limiting crop productivity worldwide. We hypothesized that it is possible to enhance drought tolerance by delaying stress-induced senescence through the stress-induced synthesis of cytokinins in crop-plants. We generated transgenic rice (Oryza sativa) plants expressing an isop...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/j.1467-7652.2010.00584.x
更新日期:2011-09-01 00:00:00
abstract::Suppression of the microsomal ω6 oleate desaturase during the seed development of soybean (Glycine max) with the 420-bp soybean FAD2-1A intron as RNAi trigger shifts the conventional fatty acid composition of soybean oil from 20% oleic and 60% polyunsaturates to one containing greater than 80% oleic acid and less than...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/j.1467-7652.2010.00573.x
更新日期:2011-09-01 00:00:00
abstract::Genetic engineering to enhance mercury phytoremediation has been accomplished by expression of the merAB genes that protects the cell by converting Hg[II] into Hg[0] which volatilizes from the cell. A drawback of this approach is that toxic Hg is released back into the environment. A better phytoremediation strategy w...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/j.1467-7652.2011.00616.x
更新日期:2011-06-01 00:00:00
abstract::Approximately 20,000 of the rice-FOX Arabidopsis transgenic lines, which overexpress 13,000 rice full-length cDNAs at random in Arabidopsis, were screened for bacterial disease resistance by dip inoculation with Pseudomonas syringae pv. tomato DC3000 (Pst DC3000). The identities of the overexpressed genes were determi...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/j.1467-7652.2010.00568.x
更新日期:2011-05-01 00:00:00
abstract::Lutein and zeaxanthin cannot be synthesized de novo in humans, and although lutein is abundant in fruit and vegetables, good dietary sources of zeaxanthin are scarce. Certain corn varieties provide adequate amounts because the ratio of endosperm β:ε lycopene cyclase activity favours the β-carotene/zeaxanthin branch of...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/j.1467-7652.2010.00554.x
更新日期:2011-04-01 00:00:00
abstract::Transcription factors have been shown to control the activity of multiple stress response genes in a coordinated manner and therefore represent attractive targets for application in molecular plant breeding. We investigated the possibility of modulating the transcriptional regulation of drought and cold responses in t...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/j.1467-7652.2010.00547.x
更新日期:2011-02-01 00:00:00
abstract::Threonine (Thr) is one of a few limiting essential amino acids (EAAs) in the animal feed industry, and its level in feed rations can impact production of important meat sources, such as swine and poultry. Threonine as well as EAAs lysine (Lys) and methionine (Met) are all synthesized via the aspartate family pathway. ...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/j.1467-7652.2010.00545.x
更新日期:2011-02-01 00:00:00
abstract::2-Acetyl-1-pyrroline (2AP), the volatile compound that provides the 'popcorn-like' aroma in a large variety of cereal and food products, is widely found in nature. Deficiency in amino aldehyde dehydrogenase (AMADH) was previously shown to be the likely cause of 2AP biosynthesis in rice (Oryza sativa L.). In this study...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/j.1467-7652.2010.00533.x
更新日期:2011-01-01 00:00:00
abstract::Genetic modification of Zn/Cd accumulation in roots and shoots for biofortification or phytoremediation is a focus of this manuscript. We expressed AtHMA4 (a P(₁B) ATPase involved in Zn and Cd transport), AtHMA4-trunc (lacking the C-terminal region) and AtHMA4-C terminus (the C-terminal region alone) in tobacco under ...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/j.1467-7652.2010.00531.x
更新日期:2011-01-01 00:00:00
abstract::Flavonoids are widely distributed in plants and play important roles in human and animal health and nutrition. Model plants with discernible flavonoid phenotypes, such as Arabidopsis seed patterning lines, are valuable tools that can provide avenues for understanding flavonoid and proanthocyanidin accumulation pattern...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/j.1467-7652.2010.00525.x
更新日期:2010-12-01 00:00:00
abstract::Coordination of multiple transgenes is essential for metabolic engineering of biosynthetic pathways. Here, we report the utilization of two bicistronic systems involving the 2A sequence from the foot-and-mouth disease virus and the internal ribosome entry site (IRES) sequence from the crucifer-infecting tobamovirus to...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/j.1467-7652.2010.00543.x
更新日期:2010-10-01 00:00:00
abstract::Plant genetic engineering can create transgenic crops with improved characteristics by introducing trait genes through transformation. Appropriate regulatory elements such as promoters and terminators have to be present in certain configurations for the transgenes to be properly expressed. Five terminators native to s...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/j.1467-7652.2010.00504.x
更新日期:2010-09-01 00:00:00
abstract::It is widely recognized that biofuel production from lignocellulosic materials is limited by inadequate technology to efficiently and economically release fermentable sugars from the complex multi-polymeric raw materials. Therefore, endoglucanases, exoglucanase, pectate lyases, cutinase, swollenin, xylanase, acetyl xy...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/j.1467-7652.2009.00486.x
更新日期:2010-04-01 00:00:00
abstract::Salicylic acid (SA) is an essential hormone for plant defence and development. SA perception is usually measured by counting the number of pathogens that grow in planta upon an exogenous application of the hormone. A biological SA perception model based on plant fresh weight reduction caused by disease resistance in A...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/j.1467-7652.2009.00468.x
更新日期:2010-02-01 00:00:00
abstract::We constructed a novel autonomously replicating gene expression shuttle vector, with the aim of developing a system for transiently expressing proteins at levels useful for commercial production of vaccines and other proteins in plants. The vector, pRIC, is based on the mild strain of the geminivirus Bean yellow dwarf...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/j.1467-7652.2009.00462.x
更新日期:2010-01-01 00:00:00
abstract::A hybrid-proline-rich protein encoding gene (CcHyPRP) has been isolated and characterized, for the first time, from the subtracted cDNA library of pigeonpea (Cajanus cajan L.) plants subjected to drought stress. Functionality of CcHyPRP has been validated for abiotic stress tolerance using the heterologous yeast and A...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/j.1467-7652.2009.00467.x
更新日期:2010-01-01 00:00:00
abstract::The production of biodegradable polymers that can be used to substitute petrochemical compounds in commercial products in transgenic plants is an important challenge for plant biotechnology. Nevertheless, it is often accompanied by reduced plant fitness. To decrease the phenotypic abnormalities of the sprout and to in...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/j.1467-7652.2009.00451.x
更新日期:2009-12-01 00:00:00
abstract::Menadione sodium bisulphite (MSB) is a water-soluble derivative of vitamin K3, or menadione, and has been previously demonstrated to function as a plant defence activator against several pathogens in several plant species. However, there are no reports of the role of this vitamin in the induction of resistance in the ...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/j.1467-7652.2009.00439.x
更新日期:2009-10-01 00:00:00
abstract::Zinc-finger nucleases (ZFNs) are artificial restriction enzymes, custom designed for induction of double-strand breaks (DSBs) at a specific locus. These DSBs may result in site-specific mutagenesis or homologous recombination at the repair site, depending on the DNA repair pathway that is used. These promising techniq...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/j.1467-7652.2009.00446.x
更新日期:2009-10-01 00:00:00
abstract::Orobanche spp. (broomrape) are parasitic plants which subsist on the roots of a wide range of hosts, including tomato, causing severe losses in yield quality and quantity. Large amounts of mannitol accumulate in this parasitic weed during development. Mannose 6-phosphate reductase (M6PR) is a key enzyme in mannitol bi...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/j.1467-7652.2009.00418.x
更新日期:2009-08-01 00:00:00
abstract::Over 3.5 million expressed sequence tags from the major cereal taxa were used to electronically mine over 176,000 putative single nucleotide polymorphisms (SNPs). The density, distribution and degree of linkage between these SNPs were compared among the different taxa. The frequency of sequence polymorphism was lowest...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/j.1467-7652.2009.00412.x
更新日期:2009-05-01 00:00:00
abstract::Advances in the genetic transformation of cereals have improved the prospects of using biotechnology for plant improvement, and a toolbox of promoters with defined specificities would be a valuable resource in controlling the expression of transgenes in desired tissues for both plant improvement and molecular farming....
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/j.1467-7652.2008.00394.x
更新日期:2009-04-01 00:00:00
abstract::Dioxin residues widely contaminate soil and agricultural products at low concentrations and may accumulate in organisms at the top of food chains owing to their physicochemical properties. In this study, we have developed novel, dioxin-inducible, reporter gene expression systems regulated by recombinant aryl hydrocarb...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/j.1467-7652.2008.00378.x
更新日期:2009-02-01 00:00:00
abstract:SUMMARY:Compared with other plant expression systems used for pharmaceutical protein production, alfalfa offers the advantage of very homogeneous N-glycosylation. Therefore, this plant was selected for further attempts at glycoengineering. Two main approaches were developed in order to humanize N-glycosylation in alfal...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/j.1467-7652.2008.00353.x
更新日期:2008-09-01 00:00:00
abstract::Expression of the cecropin A gene in rice confers resistance to the rice blast fungus Magnaporthe oryzae. In this study, a polymerase chain reaction-based suppression subtractive hybridization approach was used to generate a cDNA macroarray from the elite japonica rice (Oryza sativa L.) cultivar 'Senia'. Gene expressi...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/j.1467-7652.2008.00339.x
更新日期:2008-08-01 00:00:00
abstract::Reverse transcription-polymerase chain reaction (RT-PCR) approaches have been used in a large proportion of transcriptome analyses published to date. The accuracy of the results obtained by this method strongly depends on accurate transcript normalization using stably expressed genes, known as references. Statistical ...
journal_title:Plant biotechnology journal
pub_type: 杂志文章
doi:10.1111/j.1467-7652.2008.00346.x
更新日期:2008-08-01 00:00:00